This intestinal-flora improvement product named Refoule (Specific Microbial Extract L16Y24) was researched, analyzed and developed by the world renowned Prof. Dr. Hisatoki Komaki, currently the Honorary President and Chancellor of I.E.E.U.  (International Earth Environmental University) in USA and Japan. Prof. Dr. Hisatoki Komaki was the 1975 Nobel Prize Nominee for Medicine and Physiology from Japan.

His meticulous and continuous research was initiated originally at the request of the late Mr. Konosuke Matsushita, the Founder of Matsushita Electric Works Ltd., who after having achieved the status, success and honor of being a world renowned entrepreneur and philanthropist, embarked on his visionary search for ways that will improve human lifespan. Dr Komaki fulfilled this novel request by focusing on the important aspect of the faculty of balanced intestinal flora maintenance, hence the by-product of his research – Refoule.

It is a well known fact that there exists a hundred types of one hundred trillions (in total) of intestinal bacterial in our intestines and amongst them, there involve forty (40) types of good-natured or (beneficial) bacteria, thirty (30) types of bad-natured or (harmful) bacteria, and thirty (30) types of “wait-and-see” or “opportunistic” bacteria. As harmful bacteria, such as colon bacillus increases, our health is impaired.  As beneficial bacteria, such as lactic acid bacteria and yeast increases, it is useful and improves our health.  In short, to remain healthy, the “second Kidney”- the intestine – has to be dominated by good bacteria. To achieve that, the intestinal flora must be maintained and cultured well.

It is clear that the increase of chronic patients relates to a drop in the number of beneficial bacteria in them. It has been proven by many researchers that the predominance of harmful bacteria in bowel will badly influence the liver function, digestive function and circulatory system with ensuing constipation, smelly gas or excrement. In short, we have to maintain our bowel in beneficial-bacteria dominant condition in order to maintain optimum well-being.

Mr Komaki noticed that in our bowel, the lactic acid bacteria reproduces themselves with the help of secretion of yeast, as well as the yeast likewise propagates themselves with the help of lactic acid bacteria. That is to say both microbes (lactic acid bacteria and yeast) co-exist in proliferation with each other with the help of each other’s secretion. He also reached the conclusion that the important factor here is not the live bacteria itself but the constituent ingredients of lactic acid bacteria or yeast. The lactic acid bacteria, yeast and secretion also should not be dissolved or destroyed by gastric juices before these can exert their functions.

L16Y24 patented formulation is the specially selected 16 types of supreme strain lactic acid producing bacteria and the specially selected 24 types of supreme strain yeast cultured under the principle of beneficial co-existence and manufactured using a proprietary patented manufacturing process.

The main constituent ingredient composition of Refoule are :

The product has been marketed and distributed in Japan for more than 15 years since its first inception, with the addition of special ingredient sativumin complex (Komaki Extract) in recent times.

It is important to keep a balance between the two types of bacteria so that disease-causing harmful bacteria will not dominate over the beneficial bacteria. A stressful lifestyle, variations in weather, diet habit, medications, drug, alcohol or nicotine abuse, onset of illness, etc. will decrease the beneficial bacteria.

The secrets to good health are many – good habits, proper exercises, balanced diet and healthy lifestyle. One of the most important factors contributing to good health maintenance is the efficient absorption of nutrients in the intestines and the beneficial bacteria mentioned above are responsible for ensuring an intestinal flora balance to achieve good health.                                                               

The discovery of lactic acid bacteria was made by Prof. Elie Metchnikoff – a 1908 Nobel Prize Winner of Russian-French descent – and eventually led to the creation of numerous products harnessing the natural goodness of the various types and strains of lactic acid bacteria.

The “Theory of Longevity” written by Prof. Elie Metchnikoff created great interest and in the worldwide studies that followed, the type of bacterium found most able to survive and do good in human were included in the formulation from the meticulous research conducted by Prof. Dr. Hisatoki Komaki.

In short, lactic acid bacteria work by secreting lactic acid and antibiotic compounds which help to destroy harmful intestinal bacteria and reverse the effect of an unbalanced diet, stress, fatigue or medication, etc.

Benefits of Re foule

The beneficial properties of the supreme strains of lactic acid bacteria and yeast together with green tea extract and Komaki Extract are as follow:

1. production of enzymes such as proteases which help to digest proteins, and lipases which help to digest fat.


2. production of B vitamins which are biocatalysts in food digestion, particularly folic acid and B12.


3. production of natural antibiotics and microbial substances.


4. inhibition of the growth of 23 toxic-producing micro-organisms.


5. reduction of the possibility of bad breath, bloating, gas formation and stomach cramps.


6. reduction of occurrence of diarrhea and urinary and vaginal infection by virtue of its inhibition of gastro-intestinal and urea-pathogens.


7. enhancement of calcium metabolism and thus may be related to its hypo-cholesteremic and anti-carcinogenic effects.


8. reduction of serum cholesterol levels.


9. modifying and improving gastro-intestinal microbial balance and thus alleviate dermatitis and other skin disorders.


10. production and augmentation of immune antibodies and their function.


11. prevention of gastro-intestinal disorders which pre-dispose the individual to colon-rectal carcinogenesis.


12. improvement of the digestibility of food and efficient absorption of nutrients.


13. alleviation of lactose intolerance caused by the deficiency of enzyme lactase.


14. overall, it helps to keep the intestines healthy and the digestive system functioning well.